Transcriptome Analysis of CHO Cell Size Increase During a Fed-Batch Process
نویسندگان
چکیده
منابع مشابه
The successful transfer of a modern CHO fed-batch process to different single-use bioreactors
Introduction Nowadays, single-use bioreactors are widely accepted in pharmaceutical industry. This is based on shorter batch to batch times, reduced cleaning effort and a significantly lower risk of cross contaminations [1,2]. One large field of the application of single-use bioreactors is the seed train cultivation of mammalian cells [1]. The focus is further extended to perform state of the a...
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level: intermediAte Most biopharmaceutical production platforms are based on fed-batch cell culture protocols, which can support high volumetric productivity while maintaining low operational complexity (1). The industry is interested in developing or refining high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs (2). Although advancements in cell en...
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This report describes the building of a simulator for prediction of the dissolved oxygen concentration, the oxygen transfer rate and the concentration of carbon dioxide in a fermentation process. The steady state models were made using the linguistic equations method. The dynamic models were made using Simulink ® toolbox in the Matlab ® . At the beginning, some basics about fermentation and mic...
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Background In the biopharmaceutical industry, process development and optimization is key to produce high quality recombinant proteins at high yields. As technologies mature, pressure on cost and timelines becomes greater for delivering scalable and robust processes. Overall, process development should be viewed as a continuum from the early stages up to process validation. Here we outline a le...
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CHO cell culture high productivity relies on optimized culture medium management under fed-batch or perfused chemostat strategies enabling high cell densities. In this work, a dynamic metabolic model for CHO cells was further developed, calibrated and challenged using datasets obtained under four different culture conditions, including two batch and two fed-batch cultures comparing two differen...
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2018
ISSN: 1860-6768
DOI: 10.1002/biot.201800156